Sirtuins are members of the NAD-dependent histone deacetylase family of proteins that participate in a variety of cellular functions, including histone deacetylation, gene silencing, chromosomal stability, and aging.
SIRT2, a human homolog of the yeast SIR2 (silent information regulator-2), functions as transcriptional silencing mediator at mating-type loci, telomeres and ribosomal gene clusters.
SIRT2 expression increases dramatically during mitosis and is multiply phosphorylated at the G(2)/M transition of the cell cycle.
SIRT2 is part of a phosphorylation cascade where it is phosphorylated late in G(2), during M, and into the period of cytokinesis.
Inhibition of SIRT2 is reported to rescue alpha-synuclein toxicity and modify inclusion morphology in a cellular model of Parkinsons disease.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
