84-4052-75　［取扱停止］FasLG 100ul　315722

特徴

• Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers .
• Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes .
• FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at ~40kD upon glycosylation) that belongs to the TNF family, which also includes TNF-alpha, TRAIL, and TWEAK.
• Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 .
• Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis.
• Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites .
• FasL is also expressed in several tumor types as a mechanism to evade immune surveillance .
• Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26kD trimeric soluble form .
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.