Protein with hypoxia-induced expression in endothelial cells.
May act as a regulator of angiogenesis and modulate tumorigenesis.
Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage.
May exert a protective function on endothelial cells through an endocrine action.
It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity.
In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM).
The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion.
It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-2000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Angiopoietin-related protein 4.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa26-406 from human Angiopoietin-related protein 4.
