84-4049-53　Cyclin-dependent kinase 9 (CDK9 ) 50ul　312544

特徴

• Protein kinase involved in the regulation of transcription.
• Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP.
• This complex is inactive when in the 7SK snRNP complex form.
• Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF.
• Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g.
• TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling).
• Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis.
• P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export.
• Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3, integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.
• The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro.
• Replication stress response protein, the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage.
• In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6.
• Promotes cardiac myocyte enlargement.
• RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription.
• AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth.
• DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect.
• The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.