84-4047-85　AP-2 complex subunit beta (AP2B1) 50ul　306269

特徴

• Component of the adaptor protein complex 2 (AP-2).
• Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways.
• Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation.
• AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome.
• The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.
• Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation.
• AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis.
• AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface.
• AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules.
• AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway.
• The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins, at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif.
• The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly, clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:200-1000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.