84-4044-95　Cyclic AMP-responsive element-binding Protein 3 (CREB3) 100ul　312454

特徴

• Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR).
• Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression.
• Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency.
• Isoform 2 plays a role in the unfolded protein response (UPR).
• Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration.
• Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein.
• Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection.
• Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6).
• Isoform 3 decreases the acetylation level of histone H4.
• Isoform 3 does not promote the chemotactic activity of leukocyte cells.
• Processed cyclic AMP-responsive element-binding protein 3: acts as a transcription factor that activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response.
• Promotes cell survival against ER stress-induced apoptotic cell death during UPR.
• Activates transcription from CRE and C/EBP-containing reporter genes.
• Induces transcriptional activation of chemokine receptors.
• Activates transcription of genes required for reactivation of the latent HSV-1 virus.
• Down-regulates Tat-dependent transcription of the HIV-1 LTR by interacting with HIV-1 Tat.
• It's transcriptional activity is inhibited by CREBZF in a HCFC1-dependent manner, by the viral transactivator protein VP16 and by the HCV core protein.
• Binds DNA to the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3') and C/EBP sequences present in many viral and cellular promoters.
• Binds to the unfolded protein respons element (UPRE) consensus sequences sites.
• Binds DNA to the 5'-CCAC[GA]-3'half of ERSE II (5'-ATTGG-N-CCACG-3').
• Associates with chromatin to the HERPUD1 promoter.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.