Outer capsid protein involved in attachment and possibly entry into the host epithelial cell.
It is subsequently lost, together with VP4, following virus entry into the host cell.
The outer layer contains 780 copies of VP7, grouped as 260 trimers.
Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors.
In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Horseradish peroxidase (HRP)
Specificity：Recognizes Rotavirus A
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Recombinant protein corresponding to aa51-326 of Rotavirus A Outer capsid glycoprotein VP7
