The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2.
It binds directly to the largest luminal loop of SV2A, SV2B and SV2C.
It is then internalized by receptor-mediated endocytosis.
The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol.
After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release.
Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes Clostridium Botulinum neurotoxin type A.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Recombinant protein corresponding to aa1-436 of Clostridium Botulinum neurotoxin type A.
