84-4031-91　parvovirus B19 Capsid Protein VP2 (HRP) 50ug　327576

特徴

• Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1.
• The capsid encapsulates the genomic ssDNA.
• Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1.
• This attachment induces virion internalization predominantly through clathrin-dependent endocytosis.
• Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s).
• VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell.
• Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein.
• Exposure of nuclear localization signal probably allows nuclear import of capsids (By similarity).
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.