84-4028-28　DNAJB2 (DnaJ Homolog Subfamily B Member 2, DnaJ Protein Homolog 1, Heat Shock 40kD Protein 3, Heat Shock Protein J1, HSJ-1, HSJ1, HSPF3) (HRP) 50ug　313766

特徴

• The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions.
• The presence of the J domain defines a protein as a member of the DnaJ family.
• DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein.
• The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis.
• The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function.
• DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis.
• DnaJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus.
• Two isoforms are produced due to alternative splicing.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.