84-4022-79　Hepatitis C virus genotype 1a Genome polyprotein (HRP) 100ug　318643

特徴

• Core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding.
• Modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit.
• Also regulates many host cellular functions such as signaling pathways and apoptosis.
• Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation.
• Thought to play a role in virus-mediated cell transformation leading to hepatocellular carcinomas.
• Interacts with, and activates STAT3 leading to cellular transformation.
• May repress the promoter of p53, and sequester CREB3 and SP110 isoform 3/Sp110b in the cytoplasm.
• Also represses cell cycle negative regulating factor CDKN1A, thereby interrupting an important check point of normal cell cycle regulation.
• Targets transcription factors involved in the regulation of inflammatory responses and in the immune response: suppresses NK-kappaB activation, and activates AP-1.
• Could mediate apoptotic pathways through association with TNF-type receptors TNFRSF1A and LTBR, although its effect on death receptor-induced apoptosis remains controversial.
• Enhances TRAIL mediated apoptosis, suggesting that it might play a role in immune-mediated liver cell injury.
• Seric core protein is able to bind C1QR1 at the T-cell surface, resulting in down-regulation of T-lymphocytes proliferation.
• May transactivate human MYC, Rous sarcoma virus LTR, and SV40 promoters.
• May suppress the human FOS and HIV-1 LTR activity.
• Alters lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage.
• Core protein induces up-regulation of FAS promoter activity, and thereby probably contributes to the increased triglyceride accumulation in hepatocytes (steatosis) (By similarity).
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.