84-4021-83　human Hypoxia-inducible factor 1-alphapolyclonal (FITC) 50ug　319745

特徴

• Functions as a master transcriptional regulator of the adaptive response to hypoxia.
• Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.
• Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
• Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters.
• Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300.
• Activity is enhanced by interaction with both, NCOA1 or NCOA2.
• Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
• Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and StabilityStore product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.