TEM-type are the most prevalent beta-lactamases in enterobacteria, they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins.
TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime.
TEM-5 is capable of hydrolyzing ceftazidime.
TEM-6 is capable of hydrolyzing ceftazidime and aztreonam.
TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime.
IRT-4 shows resistance to beta-lactamase inhibitors.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP)
Specificity：Escherichia coli
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ec
Immunogen：Recombinant Escherichia coli Beta-lactamase TEM protein（24-286aa）
