Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state.
During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.
The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes.
Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.
This fusion is pH independent and occurs directly at the outer cell membrane.
The trimer of F1-F2 (F protein) probably interacts with H at the virion surface.
Upon HN binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes.
Later in infection, F proteins expressed at the plasma membrane of infected cells could mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Biotin
Specificity：Recognizes canine distemper virus.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to aa136-608 of canine distemper virus fusion glycoprotein F0 protein
