84-3997-35　cAMP-activated global transcriptional regulator CRP (Biotin) 50ug　308978

特徴

• A global transcription regulator.
• Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome.
• There are 3 classes of CRP promoters, class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP.
• Class III promoters require multiple activator molecules, including at least one CRP dimer.
• It can act as an activator, repressor, coactivator or corepressor.
• Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP.
• Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.
• High levels of active CRP are detrimental to growth.Plays a major role in carbon catabolite repression (CCR).
• CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr).
• In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity.
• Also plays a role in many other processes
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.