Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome.
There are 3 classes of CRP promoters, class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP.
Class III promoters require multiple activator molecules, including at least one CRP dimer.
It can act as an activator, repressor, coactivator or corepressor.
Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP.
Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.
High levels of active CRP are detrimental to growth.Plays a major role in carbon catabolite repression (CCR).
CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr).
In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity.
Also plays a role in many other processes
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and StabilityStore product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：E. coli
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Escherichia coli
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ec
Immunogen：Recombinant Escherichia coli cAMP-activated global transcriptional regulator CRP protein
