84-3988-89　Rabies virus Nucleoprotein 100ug　331790

特徴

• Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases.
• If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA.
• Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation.
• The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace.
• The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication.
• Protein N binds protein P in the NC through a different interaction, and can be phosphorylated.
• Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N.
• During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.