84-3955-69　Murein tetrapeptide carboxypeptidase (HRP) 50ug　324706

特徴

• Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso-Dap-D-Ala.
• To a lesser extent, can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e.
• MurNAc-tetrapeptide, UDP-MurNAc-tetrapeptide, GlcNAc-MurNAc-tetrapeptide, and GlcNAc-anhMurNAc-tetrapeptide.
• Does not act on murein sacculi or cross-linked muropeptides.
• The tripeptides produced by the LcdA reaction can then be reused as peptidoglycan building blocks, LcdA is thereby involved in murein recycling.
• Is also essential for viability during stationary phase.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.