84-3944-05 Methylated-DNA--Protein-cysteine Methyltransferase (HRP) 100ug 323768
特徴
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.
- Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme.
- This is a suicide reaction: the enzyme is irreversibly inactivated.
- Applications:Suitable for use in ELISA and Immunohistochemistry.
- Other applications not tested.
- Recommended Dilution:Optimal dilutions to be determined by the researcher.
- Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
- For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
- Aliquots are stable at -20°C for 12 months after receipt.
- Dilute required amount only prior to immediate use.
- Further dilutions can be made in assay buffer.
- Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size:100ug
- Host:rabbit
- Source Antibody:human
- Grade:Affinity Purified
- Purity:Purified by Protein G affinity chromatography.
- Form:Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP)
- Specificity:Human
- Isotype:IgG
- Calc Applications Abbrev:E IHC
- Calc Crossreactivity:Hu
- Immunogen:Recombinant human Methylated-DNA--protein-cysteine methyltransferase
- Shelf Life:1year
- Swiss Prot Number:P16455
- EU Commodity Code:30021010
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