Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP.
In addition to its role as a glycolytic enzyme, PGK-1 appears to acts as a polymerase alpha cofactor protein.
Defects in PGK1 are generally associated with chronic hemolytic anemia.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and StabilityStore product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Human, Mouse
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu Mo
Immunogen:Recombinant human Phosphoglycerate kinase 1 protein