84-3926-37　DAPK1 100ul　313047

特徴

• Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy.
• Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting.
• The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles.
• Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function.
• Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells.
• Phosphorylates STX1A and significantly decreases its binding to STXBP1.
• Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress.
• Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy.
• Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway.
• Phosphorylates RPS6, MYL9 and DAPK3.
• Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke.
• Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death.
• Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
• Isoform 2 cannot induce apoptosis but can induce membrane blebbing.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.