84-3920-64　Tyrosine-Protein kinase Blk (BLK) 50ul　338684

特徴

• Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling.
• B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors.
• Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation.
• Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.
• Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'.
• Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C.
• With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation.
• Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation.
• In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:200-1000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.