Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation.
Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains.
Catalytic activity is enhanced in the M phase.
Involved in cell proliferation.
Required to enter into S phase in response to serum stimulation.
May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1.
Probably regulates the stability of STAM2 and RASGRF1.
Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability.
The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle.
Deubiquitinates EPS15 and controles tyrosine kinase stability.
Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling.
Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules.
Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Ubiquitin C-terminal hydrolase 8 protein (1-270aa)
