84-3916-22　Probable ATP-dependent RNA helicase DDX5 (DDX5) 50ul　330584

特徴

• Involved in the alternative regulation of pre-mRNA splicing, its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner.
• Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10.
• The rate of ATP hydrolysis is highly stimulated by single-stranded RNA.
• Involved in transcriptional regulation, the function is independent of the RNA helicase activity.
• Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR.
• Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity.
• Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation.
• Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis.
• Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation.
• Acts as transcriptional repressor in a promoter-specicic manner, the function probbaly involves association with histone deacetylases, such as HDAC1.
• As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-2000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.