The closed circular ssDNA genome is first converted to a superhelical dsDNA.
Rep binds a specific region at the genome origin of replication.
It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR).
Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester.
The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase.
The polymerase synthesizes the (+) strand DNA by rolling circle mechanism.
After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication.
Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Biotin
Specificity：African cassava mosaic virus
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Recombinant African cassava mosaic virus Replication-associated protein (aa1-358).
