84-3884-65　DDX3X (ATP-Dependent RNA Helicase DDX3X, DEAD box Protein 3, X-Chromosomal, DEAD box, X Isoform, Helicase-like Protein 2, HLP2, DDX3X, DBX, DDX3) (HRP) 100ug　313189

特徴

• Multifunctional ATP-dependent RNA helicase.
• The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity.
• In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs.
• Is involved in several steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation.
• However, the exact mechanisms are not known and some functions may be specific for a subset of mRNAs.
• Involved in transcriptional regulation.
• Can enhance transcription from the CDKN1A/WAF1 promoter in a SP1-dependent manner.
• Found associated with the E-cadherin promoter and can down-regulate transcription from the promoter.
• Involved in regulation of translation initiation.
• Proposed to be involved in positive regulation of translation such as of cyclin E1/CCNE1 mRNA and specifically of mRNAs containing complex secondary structures in their 5'UTRs, these functions seem to require RNA helicase activity.
• Specifically promotes translation of a subset of viral and cellular mRNAs carrying a 5'proximal stem-loop structure in their 5'UTRs and cooperates with the eIF4F complex.
• Proposed to act prior to 43S ribosomal scanning and to locally destabilize these RNA structures to allow recognition of the mRNA cap or loading onto the 40S subunit.
• After association with 40S ribosomal subunits seems to be involved in the functional assembly of 80S ribosomes, the function seems to cover translation of mRNAs with structured and non-structured 5'UTRs and is independent of RNA helicase activity.
• Also proposed to inhibit cap-dependent translation by competetive interaction with EIF4E which can block the EIF4E:EIF4G complex formation.
• Proposed to be involved in stress response and stress granule assembly, the function is independent of RNA helicase activity and seems to involve association with EIF4E.
• May be involved in nuclear export of specific mRNAs but not in bulk mRNA export via interactions with XPO1 and NXF1.
• Also associates with polyadenylated mRNAs independently of NXF1.
• Associates with spliced mRNAs in an exon junction complex (EJC)-dependent manner and seems not to be directly involved in splicing.
• May be involved in nuclear mRNA export by association with DDX5 and regulating its nuclear location.
• Involved in innate immune signaling promoting the production of type I interferon (IFN-alpha and IFN-beta), proposed to act as viral RNA sensor, signaling intermediate and transcriptional coactivator.
• Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFNB induction, plays a role of scaffolding adapter that links IKBKE and IRF3 and coordinates their activation.
• Also found associated with IFNB promoters, the function is independent of IRF3.
• Can bind to viral RNAs and via association with MAVS/IPS1 and DDX58/RIG-I is thought to induce signaling in early stages of infection.
• Involved in regulation of apoptosis.
• May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway.
• Acts as an antiapoptotic protein through association with GSK3A/B and BIRC2 in an apoptosis antagonizing signaling complex, activation of death receptors promotes caspase-dependent cleavage of BIRC2 and DDX3X and relieves the inhibition.
• May be involved in mitotic chromosome segregation.
• Appears to be a prime target for viral manipulations.
• Hepatitis B virus (HBV) polymerase and possibly vaccinia virus (VACV) protein K7 inhibit IFNB induction probably by dissociating DDX3X from TBK1 or IKBKE.
• Is involved in hepatitis C virus (HCV) replication, the function may involve the association with HCV core protein.
• HCV core protein inhibits the IPS1-dependent function in viral RNA sensing and may switch the function from a INFB inducing to a HCV replication mode.
• Involved in HIV-1 replication.
• Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.