RP105 is a type 1 transmembrane protein of 105kD with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail.
RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs.
RP105 expression is largely restricted to immune cells including CD19+ B cells and macrophages.
Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones.
Antibody-mediated cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule CD80, revealing RP105 as a potent regulator of B-cell activation.
In this context it is important to note that RP105 is associated with MD1, and that it mediates B-cell surface expression and LPS recognition and signalling in cooperation with TLR4.
MD-1 seems to be requisite for efficient expression of RP105.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CD180. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the Internal region of human CD180.
