Golgin 45, also known as JEM-1 or Basic Leucine Zipper Nuclear Factor 1, is a 400aa protein that localizes to the lumen of the golgi apparatus.
The primary function of golgin 45 is to maintain normal golgi structure and to facilitate protein transport from the endoplasmic reticulum to the cell surface.
The latter process is regulated primarily through the interaction of golgin 45 and GRASP55, which, together, form a Rab 2 effector complex.Excluding formation of a Rab 2 complex, golgin 45 does not interact with any other golgi Rab proteins.
Golgin 45 is expressed as two isoforms, one of which localizes to the nucleus and is thought to function as a transcription factor.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Western Blot: 1:500-1:2000Immunohistochemistry: 1:100-1:300Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Golgin 45. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the N-terminal region of human Golgin 45.
