84-3843-89　ZP2 (Zona Pellucida Sperm-binding Protein 2, Zona Pellucida Glycoprotein 2, Zp-2, Zona Pellucida Protein A, ZPA) 50ug　340221

特徴

• Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene.
• The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida.
• It is also responsible for the primary block to polyspermy in mammals.
• The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin.
• After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space.
• Ovastacin cleaves ZP2 at the N-terminal, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida.
• Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases.
• Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:40,000Immunohistochemistry: 1:100-1:300Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.