Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction.
The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s).
ZNRF2 (zinc and ring finger 2), also known as RNF202, is a 242aa peripheral membrane protein that contains one RING-type zinc finger and localizes to the lysosome, as well as the endosome and the cell junction.
Expressed at high levels in brain tissue, ZNRF2 is thought to function as an E3 ubiquitin-protein ligase that may be involved in the establishment and maintenance of neuronal transmission and plasticity.
Upon DNA damage, ZNRF2 is subject to phosphorylation, probably by ATR or ATM.
Applications:Suitable for use in Immunofluorescence, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Immunohistochemistry: 1:100-1:300Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ZNRF2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the C-terminal region of human ZNRF2.
