Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism.
This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214. Species Crossreactivity: mouse and human
![PARP, cleaved (ADP-ribosyltransferase 1, ADPRT, ADPRT1, pADPRT-1, PARP, PARP-1, Poly (ADP-ribose) pol, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1, PPOL) 100ul](https://aimg.as-1.co.jp/c/84/3735/76/85133520.jpg)