MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors.
The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein.
The alpha- and beta-synuclein proteins are found primarily in brain tissue, where they are seen mainly in presynaptic terminals.
The gamma-synuclein protein is found primarily in the peripheral nervous system and retina, but its expression in breast tumors is a marker for tumor progression.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/ml for 2 hours at RT (reported)
Immunohistochemistry (Formalin/paraffin): 1:200 for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:Brain
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human pan synuclein. Species Crossreactivity: Rat.3/2/07 replace Desc1 which was deleted-add IHC imagechange grade from purified-chack off WB-WS2/27/07-Changed Applications. Applications:Western Blot: (2-4ug/ml for 2hours at RT) (not verified)Immunohistology: (Formalin/paraffin) (Ab 1:400 for 30 min at RT) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.-Changed Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.-Changed Concentration. ~0.2mg/ml-Changed Form. Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.-ET
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide derived from near N-terminal of human alpha, beta and gamma synuclein proteins.Cellular Localization: Neuronal cytosol and presynaptic terminals.
