MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membranespanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells.
CXCR4 is expressed predominantly on naïve T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation.
It is also expressed on neutrophils, monocytes and various cell lines.
Applications:Suitable for use in Flow Cytometry (Not Tested) and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot:1ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive Control: U937 or HeLa cells
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes CXCR4 (Fusin, LESTR, HUMSTR). Species Crossreactivity: Human, monkey and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide derived from the second extracellular loop of human CXCR4. Epitope: Extracellular. MW of Antigen: 40kD.
