84-3733-74　［取扱停止］Catenin, beta (BSA & Azide Free) (MaxLight 405) 100ul　C2069-50AX-ML405

特徴

• MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.
• The catenins (a, b and g) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions.
• b-catenin also binds to N-cadherin and coimmunoprecipitates with APC.
• Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between a-actinin and a-catenin.
• Increased tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
• Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
• Other applications were not tested.
• Recommended Dilutions:Immunoprecipitation (Native verified): 10ug/mg protein lysate using Protein A
• Western Blot: 2.5-5ug/ml for 2hrs at RT
• Immunohistochemistry (Formalin/paraffin): 10-20 ug/ml for 30 minutes at RT.
• Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
• Optimal dilutions to be determined by researcher.
• Positive Control: MCF-7 cells.
• Breast carcinoma.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.