Polyethylene glycol (PEG) is a family of long chain polymers attached to a glycerine backbone.
It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution.
The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs.
The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage.
Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions.
As examples, PEG-adenosine deaminase (Adagen®) is used for the treatment of severe combined immunodeficiency syndrome, PEG–asparaginase (Oncaspar®) is used for the treatment acute lymphoblastic leukemia, PEG–interferon alpha-2a (Pegasys®) is used for the treatment Hepatitis C, Branched PEG–anti-VEGF aptamer (Pegaptanib, Macugen™) is used for the treatment Macular degeneration(age-related).
An anti-PEG antibody can be used to monitor a drug’s pharmacokinetics, including distribution, metabolism and excretion.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Westerm Blot: 1:5000-1:30,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes PEG. Shows reactivity with chemical samples in Western Blot.
