Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors.
These receptors, designated TNFR1 and TNFR2, are 55kD and 75kD, respectively, and are expressed on most cell types.
Signaling through TNFR2 is confined to cells of the immune system.
Epitope: C-terminal
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 2-4ug/ml for 2hrsImmunohistochemistry (Formalin/paraffin): 1:50 for 30 min Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.
Positive Control:Pancreatic Carcinoma or HeLa Cells
Cellular Localization:Cytoplasmic
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes TNFR2. Species Crossreactivity: Human, Mouse and Rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from C-terminus of mouse TNFR2.Cellular Localization: CytoplasmicEpitope: C-terminal. MW of Antigen: 75kD
