84-3733-53　［取扱停止］CD95 (Fas) (PE) 100ul　C2442-22C-PE

特徴

• CD95, also known as Fas, is a transmembrane glycoprotein.
• It is a member of the nerve growth factor receptor/tumor necrosis factor superfamily.
• This cell surface molecule mediates receptor-triggered apoptosis.
• The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell lines.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 2-4ug/ml for 2hrs at RTImmunohistochemistry (Formalin/paraffin): 1:100 for 60 min at RT.
• Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
• Optimal dilutions to be determined by the researcher.
• Positive Control:Tonsil, prostate, Jurkat cells
• Storage and Stability:For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.