CD7 (also known as gp40, Leu9) is a member of the immunoglobulin gene super family.
It is expressed on the majority of immature and mature T-lymphocytes, and T-cell leukemia.
It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells.
Crosslinking surface CD7 positively modulates T-cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation.
CD7 associates directly with phosphoinositol-3-kinase.
CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.
Primitive T-cell neoplasms such as lymphoblastic leukemia express CD7 antigen.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml for 2 hrs at RT
Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human CD7.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant CD7 protein. Cellular Localization: Membrane. MW of Antigen: 40kD
