Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid.
Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors.
Stining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm.
Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistology (Formalin/paraffin): 1:150 for 10 min at RT Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.
Positive Control:Thyroid or medullary carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Calcitonin. Species Crossreactivity: Human, monkey, mouse, rat, canine, equine, sheep and porcine.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Ca Eq Hu Mk Mo Po Rt Sh
Immunogen：Synthetic human calcitonin peptide (aa1-32). Cellular Localization: Cytoplasmic.
