Synaptophysin labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells in the gastrointestinal tract and of gastric parietal cells.
Neurons in the brain, spinal cord, and retina are also labeled.
Anti-synaptophysin reacts with neuroendocrine neoplasms of neural as well as epithelial types e.g.
neuroblastomas, ganglioneuroblastomas.
ganglioneuromas, pheo-chromocytomas, chromaffin, and non-chromaffin paragangliomas.
Of the epithelial types include pituitary adenomas, islet cell neoplasms, medullathyroid carcinomas, parathyroid adenomas, carcinoids of the bronchopulmonary and gastro-intestinal tracts and neuroendocrine carcinomas of the skin.
In combination with anti-chromogranin A and anti-NSE, anti-synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 5-10ug/ml for 2 hours at RT
Immunohistochemistry (formalin/paraffin): 5-10ug/ml for 30 min at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined bythe researcher.
MW of Antigen: 38kD
Epitope: C-terminal
Positive Control:IMR-5 cells.
Pancreas or Pheochromocytoma or GI tumor.
Cellular Localization:Cytoplasmic
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at-20°C or colder.
Aliquots are stable for at least 12 months at-20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Reacts with neuroendocrine neoplasms of neural as well as epithelial types. In combination with anti-chromogranin A and anti-NSE, very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms. Species Crossreactivity: Human and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide from C-terminus of human synaptophysin.
