ROC1 and ROC2 are the two highly conserved RING finger proteins that are homologous to APC11, a subunit of the anaphase-promoting complex(APC).
ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit.
ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1-and E2-dependent manner.
ROC1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin1 to catalyze ubiquitin polymerization.
MW of Antigen:13kD
Epitope: aa97-108
Applications and Recommended Dilutions:Immunoprecipitation (Native verified) (Use Protein A; Ab at 10ug/mg protein lysate) Western Blotting (5-10ug/ml for 2hrs at RT)
The optimal dilution for a specific application should be determined by the investigator.
Positive Control: HeLa cells.
Tonsil.
Cellular Localization: Nuclear
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at-20°C or colder.
Aliquots are stable for at least 12 months at-20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes ROC1. Species Crossreactivity: human and mouse.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：A peptide corresponding to amino acid residue 97-108 (CPLDNREWEFQKYGH) from C-terminus of the human ROC1.
