CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membranespanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells.
CXCR4 is expressed predominantly on naïve T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation.
It is also expressed on neutrophils, monocytes and various cell lines.
Applications:Suitable for use in Flow Cytometry (Not Tested) and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot:1ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive Control: U937 or HeLa cells
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at-20°C.
Aliquots are stable for at least 12 months at-20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes CXCR4 (Fusin, LESTR, HUMSTR). Species Crossreactivity: Human, monkey and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide derived from the second extracellular loop of human CXCR4. Epitope: Extracellular. MW of Antigen: 40kD.
