PMP-22 (Peripheral Myelin Protein) is a glycoprotein expressed in the compact myelin of the peripheral nervous system.
In the peripheral nervous system, PMP-22 is produced by myelinating Schwann cells and is coexpressed with the genes for myelin basic protein (MBP) and protein P0 during nerve development and regeneration.
However, Schwannoma cells not associated with peripheral nerve will be negative.
Alterations in the level of this protein cause several genetic human diseases.
If the protein is duplicated, patients develop Charcot- Marie-Tooth disease.
If one copy of the gene is deleted, they suffer from the inherited tendency to pressure palsies.
PMP-22 has recently been reported in high-grade osteosarcoma.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: (Formalin/paraffin): 1:12.5-1:25 for 60 min.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PMP-22.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide from near C-terminal of human PMP-22. Epitope: Near C-terminus. Cellular Localization: Cytoplasmic, Molecular Weight of Antigen: 22kD.
