84-3733-22 [取扱停止]Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) (HRP) 100ul F4565-09-HRP
特徴
Flk-1/KDR /VEGFR2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended dilutions:Immunohistochemistry (Formalin/paraffin): 1:25.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control: Angiosarcoma
Cellular Localization: Cytoplasmic
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes Flk-1. Species Crossreactivity: Human, Mouse and Rat.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:A synthetic peptide derived from the C-terminal of human Flk-1
Shelf Life:1year
EU Commodity Code:30021010
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