ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition.
The ER is an important regulator of growth and differentiation in the mammary gland.
Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g.
tamoxifen) therapy
Applications:Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 10ug/mg protein lysate Use Protein AWestern Blot: 2.5-5ug/ml for 2hrs at RTImmunohistochemistry: (Formalin/paraffin): 1:500 for 20 minutes at RT using the LP system, for 30 minutes at RT Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by researcher.
Positive Control:MCF-7 cells.
Breast carcinoma
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Estrogen Receptor (ER). Species Crossreactivity: Mouse and hamster.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hm Hu Mo
Immunogen：A synthetic peptide derived from the C-terminus of human estrogen receptor, alpha protein Epitope: C-terminal. Cellular Localization: Nuclear. MW of Antigen: 67kD
