Cyclin D1 gene is identical with the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma.
Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms.
About 50-70% of mantle cell lymphomas (MCL) contain a t (11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1.
Cyclins were originally identified as proteins whose levels fluctuated during the cell cycle.
Subsequent studies have shown the cyclins control entry into different phases of the cycle cell by regulating the activity of cyclin-dependent kinases (CDKs).
Mitotic or G2 cyclins regulate entry into M phase, whereas G1 cyclins regulate entry into S phase.
Cyclin D1, a G1 cyclin, is essential for control at the G1/S transition (START in yeast), as it regulates the activity of CDK4 and CDK6 protein kinases.
Translocation of the gene encoding cyclin D1, the bcl-1 oncogene, is associated with human B-lymphocytic malignancy, suggesting that alteration of cell cycle progression may be oncogenic.
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase Assay.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation (Native and denatured): 10ug/mg protein lysate.
Co-precipitates Cdk4
Western Blot: 5-10ug/ml for 2hrs at RTOptimal dilutions to be determined by the researcher.
Positive Control:MAD109 cells.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human cyclin D1 (Mr 36kD) and shows no crossreaction with cyclin D2 or D3. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Sythetic peptide corresponding to aa 285-295 (Cys-TPTDVRDVDI) from the C-terminal of human cyclin D1 (KLH). Cellular Localization: Nuclear. MW of Antigen: 36kD.
