Stat3 protein is a transcription factor expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1.
Stat3 binds to DNA as either a homodimer or as a heterodimer with Stat1.
Stat3 interacts with APRE (acute phase response element) and SIE (c-sis inducible element).
Applications:Suitable for use in Gel Supershift and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 5-10ug/ml for 2hrs at RT
Optimal dilution determined by the researcher.
Positive Control:Jurkat cells
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human STAT 3 (Signal Transducers and Activators of Transcription 3). Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide from C-terminus of human Stat3. MW of Antigen: 89kD. Epitope: C-terminal. Cellular Localization: Nuclear
