Stat3 protein is a transcription factor expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1.
Stat3 binds to DNA as either a homodimer or as a heterodimer with Stat1.
Stat3 interacts with APRE (acute phase response element) and SIE (c-sis inducible element).
Applications:Suitable for use in Gel Supershift and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 5-10ug/ml for 2hrs at RT
Optimal dilution determined by the researcher.
Positive Control:Jurkat cells
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human STAT 3 (Signal Transducers and Activators of Transcription 3). Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide from C-terminus of human Stat3. MW of Antigen: 89kD. Epitope: C-terminal. Cellular Localization: Nuclear
