MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Due to a concerted effort to identify biomarkers for lung and colon carcinomas by genome-wide transcriptional profiling, the identification and cloning of one such gene as well as two additional closely related genes was achieved.
Due to the strong sequence homology to the C.
elegans a novel gene was named URP1, for Unc-112 related protein.
Another novel related gene, URP2 and the previously discovered MIG-2 gene was also identified.
Transcriptional analysis shows that only URP1 is significantly differentially regulated, being over-expressed in 70% of the colon carcinomas and 60% of the lung carcinomas tested.
Quantification of URP1 expression by qRT-PCR showed up-regulation of the gene by 60-fold in lung tumors and up to nearly 6-fold in colon tumors.
Northern blot analysis of URP1 indicates that normal expression is restricted to neuromuscular tissues.
In contrast, the expression of URP2 appears to be confined primarily to tissues of the immune system.
URP1 has the potential to be one of the most important prognostic and diagnostic markers of colon or lung cancer.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes residues aa643-660 [VHEYIGGYIFLSTRSKDQ] of the 77kD human Unc-112-related protein 1. Species sequence homology: (100%): mouse, rat, canine, bovine, chimpanzee, Xenopus (Almost all species)
