84-3724-71 Oct 3/4 (Octamer-Binding Transcription Factor 3/4) (MaxLight 650) 100ul 301433-ML650
特徴
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells.
A moderate increase in expression causes differentiation into primitive endoderm and mesoderm.
In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:100-1:250Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity:Recognizes human Oct 3/4.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to an internal sequence of human Oct 3/4.
Shelf Life:1year
EU Commodity Code:30021010
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